Improving Results from Touch DNA Evidence with Optimized Direct Polymerase Chain Reaction (PCR) Methods, 2020-2022 (ICPSR 38910)
Principal Investigator(s): View help for Principal Investigator(s)
Abigail S. Bathrick, Bode Technology;
Anna C. Salmonsen, Bode Technology;
Jonathan M. Davoren, Bode Technology
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Summary View help for Summary
Direct polymerase chain reaction (PCR) is a DNA processing method in which a sample is added directly to an amplification reaction without prior purification or quantification and has been identified as a method that may improve genotyping data obtained from low-yield touch DNA samples. The goal of the project was to generate data in support of a re-evaluation of the Federal Bureau of Investigation's (FBI) Quality Assurance Standard (QAS) 9.4 and the 2018 Forensic Science Technology Working Group (TWG) operational requirements.
The project was performed in two phases. Phase I examined the direct PCR-compatible collection methods in conjunction with mock touch DNA evidence samples on a variety of substrates. Phase II examined direct PCR of touch DNA samples that were stored at room temperature for up to six months after collection and samples that were re-sampled after initial processing. Direct PCR was performed using GlobalFiler and PowerPlex Fusion 6C amplification methods that were already validated for standard casework processing.
The technical summary for this project can be downloaded from the National Institute of Justice project page: https://nij.ojp.gov/library/publications/improving-results-touch-dna-evidence-optimized-direct-pcr-methods.